01817

Fluka

 

Actinomycin D

BioChemika, ≥98.0% (HPLC), from Streptomyces sp.

Synonym:Actinomycin C1, Actinomycin IV, Dactinomycin
CAS Number:50-76-0
Linear Formula:C62H86N12O16
Molecular Weight:1255.42
Beilstein Registry Number:605235
EC Number:200-063-6
MDL number:MFCD00005033

Description

Biochem/physiol ActionsAn antineoplastic antibiotic that inhibits cell proliferation by forming a stable complex with DNA and blocking the movement of RNA polymerase which interferes with DNA-dependent RNA synthesis. Induces apoptosis. Potent antitumor agent. For cell culture applications, actinomycin D is used as a selection agent and is used in banding techniques to differentiate between different regions of chromosomes.
Other NotesInhibits DNA-dependent RNA synthesis. DNA-binding drug1,2,3

Properties

biological source from Streptomyces sp.
product lineBioChemika
assay≥98.0% (HPLC)
color red, powder
mp251-253
solubilityacetonitrile: 10 mg/mL, clear, orange to red
storage temp.2-8°C

Safety

Personal Protective EquipmentEyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges
Hazard CodesT+
Risk Statements28
Safety Statements22-28-36/37-45
RIDADRUN3462 6.1/PG 2
WGK Germany3
RTECSAU1575000

Related Products

Replaced byA1410, Actinomycin D

References

Cited Reference1. Y. Hashimoto Meth. Enzymol. 121, 817, (1986) Abstract
 2. R.M. Wadkins, T.M. Jovin Biochemistry 30, 9469, (1991) Abstract
 3. Y. Maeda et al. J. Mol. Biol. 215, 321, (1990) Abstract
referenceWadkins, R.M., Actinomycin D binding to single-stranded DNA: sequence specificity and hemi-intercalation model from fluorescence and 1H NMR spectroscopy. J. Mol. Biol. 262, 53, (1996)
 Hu, B., et al., Kasabach-Merritt syndrome-associated kaposiform hemangioendothelioma successfully treated with cyclophosphamide, vincristine, and actinimycin D. J. Pediatr. Hematol. Oncol. 20, 567, (1998)
 Cohen, S.M., et al., Identification of chromosomal bands replicating early in the S phase of normal human fibroblasts. Exp. Cell Res. 254, 321, (1998)
 Glynn, J.M., et al.,, Apoptosis induced by Actinomycin D, Camptothecin or Aphidicolin can occur in all phases of the cell cycle. Biochem. Soc. Trans. 20, 84S, (1992)
 Verma, R.S., Babu, A. Manual of Basic Techniques New York, NY , (1989)
 Chen, H., et al., DNA bending and unwinding associated with actinomycin D antibiotics bound to partially overlapping sites on DNA. J. Mol. Biol. 258, 457, (1996)
MerckMerck 13,2828
BeilsteinBeil. 27,IV,9642
 Aldrich MSDS 1, 34:A / Corp MSDS 1 (1), 62:B / RegBook 1 (2), 2651:C / Sax 6, 134 / Sigma FT-IR 1 (1), 610:D / Structure Index 1, 417:B:6